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O-Antigen modifications providing antigenic diversity of Shigella flexneri and underlying genetic mechanisms

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Abstract

O-Antigens (O-specific polysaccharides) of Shigella flexneri, a primary cause of shigellosis, are distinguished by a wide diversity of chemical modifications following the oligosaccharide O-unit assembly. The present review is devoted to structural, serological, and genetic aspects of these modifications, including O-acetylation and phosphorylation with phosphoethanolamine that have been identified recently. The modifications confer the host with specific immunodeterminants (O-factors or O-antigen epitopes), which accounts for the antigenic diversity of S. flexneri considered as a virulence factor of the pathogen. Totally, 30 O-antigen variants have been recognized in these bacteria, the corresponding O-factors characterized using specific antibodies, and a significant extension of the serotyping scheme of S. flexneri on this basis is suggested. Multiple genes responsible for the O-antigen modifications and the resultant serotype conversions of S. flexneri have been identified. The genetic mechanisms of the O-antigen diversification by acquisition of mobile genetic elements, including prophages and plasmids, followed occasionally by gene mobilization and inactivation have been revealed. These findings further our understanding of the genetics and antigenicity of S. flexneri and assist control of shigellosis.

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Abbreviations

GalA:

galacturonic acid

GalNAc:

2-acetamido-2-deoxygalactose

GlcNAc:

2-acetamido-2-deoxyglucose

IS:

insertion sequence

LPS:

lipopolysaccharide

PEtN:

phosphoethanolamine

Rha:

rhamnose

Sf:

Shigella flexneri

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    Y. A. Knirel, S. N. Senchenkova, A. V. Perepelov & A. S. Shashkov

  2. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, P.O. Box 5, Changping, Beijing, 102206, China

    Qiangzheng Sun & Jianguo Xu

Authors
  1. Y. A. Knirel
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  2. Qiangzheng Sun
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  3. S. N. Senchenkova
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  4. A. V. Perepelov
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  5. A. S. Shashkov
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  6. Jianguo Xu
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Correspondence to Y. A. Knirel, Qiangzheng Sun or Jianguo Xu.

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Published in Russian in Biokhimiya, 2015, Vol. 80, No. 7, pp. 1072-1087.

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Knirel, Y.A., Sun, Q., Senchenkova, S.N. et al. O-Antigen modifications providing antigenic diversity of Shigella flexneri and underlying genetic mechanisms. Biochemistry Moscow 80, 901–914 (2015). https://doi.org/10.1134/S0006297915070093

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